FLUORESCENT-PROTEIN STAINING OF NUCLEOLI IN MONOLAYER CELL CULTURES: DETERMINANTS AND SIGNIFICANCE

Propagation of goat pox virus on monolayer cell cultures

In situ trypan blue staining of monolayer cell cultures for permanent fixation and mounting.

Trypan Blue is one of the most commonly used dyes for assessing cell viability by dye exclusion assays. Dye exclusion assays are based on the principle that an intact cell membrane is necessary for the exclusion of certain dyes. Some other dyes used for this purpose include safranin, eosin, Congo Red, erythrocin, nigrosin and Alcian Blue (5). Pappenheimer (4) was the first to describe the use o...

etiology, significance, diagnosis and treatment of bruxiam

چکیده ندارد.

Nonimmune Fluorescent Protein Staining of Neoplasms

In 1952 Weiler demonstrated with ultraviolet microscopy that anti-rat liver microsomal and mitochondrial rabbit globulin conjugated with fluorescein isocyanate became selectively attached to normal and hyperplastic rat liver but not to paradimethylamino-azobenzol-induced hepato-carcinoma within such livers. In 1956 he demonstrated similar antigenic differences between normal hamster kidney and ...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...